Part:BBa_K1847012:Design

placUV5-lacO-lldRO1-R2oDNA-lldRO2

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We designed three hybrid promoters combining P_lac or P_lacUV5, lacO and lldRO1 and lldRO2. Part:BBa_K1847010, Part:BBa_K1847011 and Part:BBa_K1847012. In Part:BBa_K1847010 and Part:BBa_K1847011 we kept the original architecture of the P_lldR leaving the operators and substituting the wild-type promoter by P_lac and lacO. The distance in nucleotides between the two lldR operator sites differs in 5 nucleotides. In Part:BBa_K1847012 the lldR wild-type promoter was substituted by a biologically neutral spacer and P_lac and lacO were located in front of the P_lldR.

Source

Synthetic synthesis